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(Plasmid #53086)


Item Catalog # Description Quantity Price (USD)
Plasmid 53086 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pPD 49.83
  • Backbone size w/o insert (bp) 3648
  • Total vector size (bp) 5724
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    deleted native signal sequence, deleted C-terminal domain,FG/AA WNL/AAA
  • Entrez Gene
    Mfge8 (a.k.a. AA408458, AI325141, MFG-E8, MP47, Mfgm, P47, SED1, lactadherin)
  • Promoter hsp-16.41
  • Tags / Fusion Proteins
    • GFP (w/ introns) (N terminal on insert)
    • signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer hsp-16
  • 3′ sequencing primer unc-54
  • (Common Sequencing Primers)

Terms and Licenses

Depositor Comments

Mutations in Lactadherin described in

C. Shao, V.A. Novakovic, J.F. Head, B.A. Seaton, G.E. Gilbert
Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif
J. Biol. Chem., 283 (2008), pp. 7230–7241

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM656 was a gift from Ding Xue (Addgene plasmid # 53086 ; ; RRID:Addgene_53086)
  • For your References section:

    CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells. Mapes J, Chen YZ, Kim A, Mitani S, Kang BH, Xue D. Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 10.1016/j.cub.2012.05.052. Epub 2012 Jun 21. 10.1016/j.cub.2012.05.052 PubMed 22727702