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(Plasmid #53107)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 53107 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Serotonin receptor 1A
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    5-HT1A (a.k.a. Dmel_CG16720, 5-HT-dro2A, 5-HT1ADro, 5-HT[1A]Dro, 5-HT[[1ADro]], 5-HT[[1A]], 5-HT[[1A]]DRO, 5HT-R2A, 5HT-dro2A, 5HT-drp[[2A]], 5HT1A, 5HT[[1ADro]], 5htdro2a, CG16720, DRO2A, Dm5HTdro2A, Dmel\CG16720, d5-HT1A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT1A. It was cloned from transgenic fly that has UAS-d5-HT1A (Bloomington #27630) and can be considered a functional variant.

Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18ST-d5-HT1A was a gift from Leslie Vosshall (Addgene plasmid # 53107)
  • For your References section:

    Small molecule drug screening in Drosophila identifies the 5HT2A receptor as a feeding modulation target. Gasque G, Conway S, Huang J, Rao Y, Vosshall LB. Sci Rep. 2013;3:srep02120. doi: 10.1038/srep02120. 10.1038/srep02120 PubMed 23817146