This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pME18ST-d5-HT2A
(Plasmid #53109)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53109 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5HT2A
  • Alt name
    serotonin receptor 2, isoform A
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    5-HT2 (a.k.a. Dmel_CG1056, 5-HT2Dro, 5-HT[2]Dro, 5-HT[[2Dro]], 5-HT[[2]], 5-HT[[2]]Dro, 5-ht2Dro, 5HT-R82A, 5HT-dro[[1]], 5HT2-Dro, 5HT2Dro, 5HT[[2Dro]], CG1056, Dm5HT2, Dmel\CG1056, d5-HT2, l(3)82CDb)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pcDL-F (5'-GTTGCCTTTACTTCTAGGCCT-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT2A. It was cloned from transgenic fly that has UAS-d5-HT2A (Bloomington #24504) and can be considered a functional variant.

Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18ST-d5-HT2A was a gift from Leslie Vosshall (Addgene plasmid # 53109)
  • For your References section:

    Small molecule drug screening in Drosophila identifies the 5HT2A receptor as a feeding modulation target. Gasque G, Conway S, Huang J, Rao Y, Vosshall LB. Sci Rep. 2013;3:srep02120. doi: 10.1038/srep02120. 10.1038/srep02120 PubMed 23817146