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pBS-I-Sce1-slc6a3[11kb]:egfp
(Plasmid #53240)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53240 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 14959
  • Modifications to backbone
    I-Sce1 addition
  • Vector type
    cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAT-GFP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    12737
  • Tag / Fusion Protein
    • GFP

Resource Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-I-Sce1-slc6a3[11kb]:egfp was a gift from Edward Burton (Addgene plasmid # 53240 ; http://n2t.net/addgene:53240 ; RRID:Addgene_53240)
  • For your References section:

    Cis-acting elements responsible for dopaminergic neuron-specific expression of zebrafish slc6a3 (dopamine transporter) in vivo are located remote from the transcriptional start site. Bai Q, Burton EA. Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12. 10.1016/j.neuroscience.2009.09.014 PubMed 19755139