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THBS1-bio-His
(Plasmid #53417)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53417 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6618
  • Total vector size (bp) 10148
  • Modifications to backbone
    Rat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    THBS1
  • Alt name
    Thrombospondin-1 precursor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3530
  • Entrez Gene
    THBS1 (a.k.a. THBS, THBS-1, TSP, TSP-1, TSP1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • rat CD4 d3+4 (C terminal on backbone)
    • enzymatic biotinylation sequence (C terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was chemically synthesised.
  • Terms and Licenses

Depositor Comments

Article ref 147

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    THBS1-bio-His was a gift from Gavin Wright (Addgene plasmid # 53417)
  • For your References section:

    A human platelet receptor protein microarray identifies FcepsilonR1alpha as an activating PEAR1 ligand. Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright GJ. Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946. 10.1074/mcp.M114.046946 PubMed 25713122