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pcdna3 Flag JIP3b
(Plasmid #53458)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53458 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcdna3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 10934
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase 8 interacting protein 3
  • Alt name
    Jip3b
  • Alt name
    Mapk8ip3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5488
  • GenBank ID
    AF178637.1
  • Entrez Gene
    Mapk8ip3 (a.k.a. BB120594, D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 Flag JIP3b was a gift from Roger Davis (Addgene plasmid # 53458 ; http://n2t.net/addgene:53458 ; RRID:Addgene_53458)
  • For your References section:

    Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Kelkar N, Gupta S, Dickens M, Davis RJ. Mol Cell Biol. 2000 Feb;20(3):1030-43. PubMed 10629060