pJL017
(Plasmid #53532)

• Purpose: This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene
• Depositing Lab: James Fleckenstein
• Publication: Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1038/nprot.2009.87. Epub 2009 Jul 2.

Backbone

• Vector backbone: pBAD/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 11268
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: see detailed protocol Nature Protocols
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: etpBA
• Species: E. coli
• Insert Size (bp): 7200
• GenBank ID: AAX13508.1 AAX13509.2
• Promoter: pBAD
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • 6His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pBAD forward
• 3′ sequencing primer: pBAD reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a minor mutation in etpB - E64G. This mutation should not affect function.