pJL030
(Plasmid #53533)

• Purpose: supplies EtpC required for optimal EtpA expression on compatible plasmid
• Depositing Lab: James Fleckenstein
• Publication: Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1038/nprot.2009.87. Epub 2009 Jul 2.

Backbone

• Vector backbone: pACYC184
• Backbone size w/o insert (bp): 3719
• Total vector size (bp): 5567
• Vector type: Bacterial Expression ; pACYC184

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: etpC
• Species: E. coli
• Insert Size (bp): 1900
• GenBank ID: AY920525.2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NruI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a L to M mutation in etpC. This mutation should not affect function.