pJY019
(Plasmid #32999)

• Depositing Lab: James Fleckenstein
• Publication: Fleckenstein et al Infect Immun. 2006 Apr . 74(4):2245-58.

Backbone

• Vector backbone: pBAD/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4094
• Modifications to backbone: none
• Vector type: Bacterial Expression ; n/a

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: see Nature Protocols Purification of recombinant high molecular weight two-partner secretion proteins from Escherichia coli 2009 vol. 4 (7) pp. 1083-92
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: etpBAC locus
• Species: E. coli
• Insert Size (bp): 9402
• GenBank ID: AY920525.2
• Promoter: pBAD
• Tag / Fusion Protein:
  • myc-6His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function.