pCS-H2B-citrine
(Plasmid
#53752)
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PurposeContains fusion of human Histone H2B to citrine.
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 53752-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 53752-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
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Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehistone H2B
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Alt namehistone cluster 2, H2be
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SpeciesH. sapiens (human)
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Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter simian CMV IE94
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Tag
/ Fusion Protein
- citrine (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byH2B was obtained from Dr. Reinhard Koster.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was created using the QuikChange protocol to perform site directed mutagenesis to make Q69M mutation to convert EYFP of pCS-H2B-EYFP (Addgene plasmid # 53746) into citrine.
Use NotI/SP6 for transcription.
DNA (Catalog # 53752-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 53752-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS-H2B-citrine was a gift from Sean Megason (Addgene plasmid # 53752 ; http://n2t.net/addgene:53752 ; RRID:Addgene_53752) -
For your References section:
In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112
Map uploaded by the depositor.
