PurposecRNA probe to detect the slc6a3 transcript
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53764||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2259
- Total vector size (bp) 3639
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Terms and Licenses
- Not Available to Industry
The insert contains S38F mutations P78L, they are not believed to effect the function of the plasmid. The clone has been successfully used
for making a probe that works well for Northern and RNA ISH (Bai 2009,
https://www.ncbi.nlm.nih.gov/pubmed/19755139), but it cannot be used for RNase protection because of the mutations. The clone only contains part of the ORF of DAT and should not be used for over-expression experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:slc6a3 probe was a gift from Edward Burton (Addgene plasmid # 53764 ; http://n2t.net/addgene:53764 ; RRID:Addgene_53764)
For your References section:Cis-acting elements responsible for dopaminergic neuron-specific expression of zebrafish slc6a3 (dopamine transporter) in vivo are located remote from the transcriptional start site. Bai Q, Burton EA. Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12. 10.1016/j.neuroscience.2009.09.014 PubMed 19755139