|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54109||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameMouse Histone H1
SpeciesM. musculus (mouse)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
M. musculus (mouse) Histone H1 with A190G mutation relative to reference sequence (NM_008197.3). Mutation should not affect function.
Excitation = 488; Emission = 509. Localization data: Nucleus/Histones
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-H1-10 was a gift from Michael Davidson (Addgene plasmid # 54109 ; http://n2t.net/addgene:54109 ; RRID:Addgene_54109)