|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54318||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationS11P, G42R, and E62D
Entrez GeneZYX (a.k.a. ESP-2, HED-2)
- Promoter CMV
/ Fusion Protein
- mEmerald (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Excitation = 487; Emission = 509.
Localization: Zyxin - Focal Adhesions
S11P, G42R and E62D mutations in human zyxin compared to reference sequence (NM_003461). These mutations should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Zyxin-C-14 was a gift from Michael Davidson (Addgene plasmid # 54318 ; http://n2t.net/addgene:54318 ; RRID:Addgene_54318)