LII ins 2-3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54379||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepAMP (Genart)
Vector typecloning vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameinsulator sequence, dummy 2-3
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
LII/LIV dummy, contain 2 repeats of a palindromic 16 bp sequence. These nucleotides have been described to act as an insulator sequence in plants, thus preventing the influence of neighboring promoters on transcriptional activation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LII ins 2-3 was a gift from Martin Parniske (Addgene plasmid # 54379 ; http://n2t.net/addgene:54379 ; RRID:Addgene_54379)
For your References section:A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M. PLoS One. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218. eCollection 2014. 10.1371/journal.pone.0088218 PubMed 24551083