CyclinB-GFP
(Plasmid
#54465)
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PurposeCyclinB-GFP in pIVT vector for in vitro transcription
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 54465-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 54465-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIVT
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Backbone manufacturerCarmen Williams (Addgene plasmid #32374)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCyclinB
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Alt namepOD021
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SpeciesM. musculus (mouse)
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MutationK67E and I150T mutations compared to GenBank reference sequence NP_114172.1
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 54465-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 54465-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CyclinB-GFP was a gift from Michael Lampson (Addgene plasmid # 54465 ; http://n2t.net/addgene:54465 ; RRID:Addgene_54465) -
For your References section:
Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I. Davydenko O, Schultz RM, Lampson MA. J Cell Biol. 2013 Jul 22;202(2):221-9. doi: 10.1083/jcb.201303019. Epub 2013 Jul 15. 10.1083/jcb.201303019 PubMed 23857768