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Purpose(Empty Backbone) Localization: Actin, Excitation: 399, Emission: 456
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 54602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonemTagBFP2-Lifeact
- Backbone size (bp) 4800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- Lifeact (C terminal on backbone)
- mTagBFP2 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
. Excitation = 399; Emission = 456
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTagBFP2-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54602 ; http://n2t.net/addgene:54602 ; RRID:Addgene_54602) -
For your References section:
An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV. PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8. 10.1371/journal.pone.0028674 PubMed 22174863