PurposeLocalization: Golgi/ER Transport, Excitation: 462, Emission: 492
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55517||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
MutationdeltaK VSVG ts045
- Promoter CMV
/ Fusion Protein
- mTFP1 (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
deltaK VSVG ts045. Excitation = 462; Emission = 492
Addgene sequencing result finds L394S, F440ins, and K511N in M11048.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mTFP1-VSVG-16 was a gift from Michael Davidson (Addgene plasmid # 55517 ; http://n2t.net/addgene:55517 ; RRID:Addgene_55517)
For your References section:Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE. BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. 10.1186/1741-7007-6-13 PubMed 18325109