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dEos-Vinculin-14
(Plasmid #57568)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 57568 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    dEos
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    NM_003373.3
  • Species
    H. sapiens (human)
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
  • Promoter CMV
  • Tag / Fusion Protein
    • dEos

Cloning Information

Resource Information

Depositor Comments

. Excitation = 505 / 569; Emission = 516 / 581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dEos-Vinculin-14 was a gift from Michael Davidson (Addgene plasmid # 57568 ; http://n2t.net/addgene:57568 ; RRID:Addgene_57568)
  • For your References section:

    Imaging intracellular fluorescent proteins at nanometer resolution. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF. Science. 2006 Sep 15;313(5793):1642-5. Epub 2006 Aug 10. 10.1126/science.1127344 PubMed 16902090