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pRK-HA-TRAF5
(Plasmid #58315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58315 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 58315-D50 50 μg of DNA in Tris buffer $495
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DNA 58315-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 4754
  • Total vector size (bp) 6430
  • Modifications to backbone
    HA tag is inserted before ClaI site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF receptor associated factor 5
  • Alt name
    TRAF5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1676
  • GenBank ID
    NM_011633 BC012702
  • Entrez Gene
    Traf5
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer RK5R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mouse TRAF5 cDNA was obtained from cDNA clone MGC-13966

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK-HA-TRAF5 was a gift from Ying Zhang (Addgene plasmid # 58315 ; http://n2t.net/addgene:58315 ; RRID:Addgene_58315)

  • For your References section:

    Ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4) by Smad ubiquitination regulatory factor 1 (Smurf1) regulates motility of breast epithelial and cancer cells. Wang X, Jin C, Tang Y, Tang LY, Zhang YE. J Biol Chem. 2013 Jul 26;288(30):21784-92. doi: 10.1074/jbc.M113.472704. Epub 2013 Jun 11. 10.1074/jbc.M113.472704 PubMed 23760265
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