Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44032||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerShao-Cong Sun
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert nameTNF receptor-associated factor 3
SpeciesH. sapiens (human)
Entrez GeneTRAF3 (a.k.a. CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1, RNF118)
/ Fusion Protein
- 1xHA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that hTRAF3 insert contains a S393G mutation when compared to GenBank entry NP_663777.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-HA-hTRAF3 was a gift from Shao-Cong Sun (Addgene plasmid # 44032 ; http://n2t.net/addgene:44032 ; RRID:Addgene_44032)
For your References section:Regulation of the NF-kappaB-inducing kinase by tumor necrosis factor receptor-associated factor 3-induced degradation. Liao G, Zhang M, Harhaj EW, Sun SC. J Biol Chem. 2004 Jun 18;279(25):26243-50. Epub 2004 Apr 14. 10.1074/jbc.M403286200 PubMed 15084608
Map uploaded by the depositor.