pEGFP-TRAF4
(Plasmid
#58318)
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PurposeEGFP-tagged mouse TRAF4
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58318-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58318-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6112
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTNF receptor associated factor 4
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Alt nameTRAF4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1412
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GenBank IDNM_009423 BC020455
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Entrez GeneTraf4 (a.k.a. A530032M13Rik, CART1, msp2)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymouse TRAF4 cDNA was obtained from cDNA clone IMAGE 3598556
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 58318-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58318-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-TRAF4 was a gift from Ying Zhang (Addgene plasmid # 58318 ; http://n2t.net/addgene:58318 ; RRID:Addgene_58318) -
For your References section:
Ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4) by Smad ubiquitination regulatory factor 1 (Smurf1) regulates motility of breast epithelial and cancer cells. Wang X, Jin C, Tang Y, Tang LY, Zhang YE. J Biol Chem. 2013 Jul 26;288(30):21784-92. doi: 10.1074/jbc.M113.472704. Epub 2013 Jun 11. 10.1074/jbc.M113.472704 PubMed 23760265
Map uploaded by the depositor.
