Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMaster1
(Plasmid #58525)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58525 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    neo
  • Insert Size (bp)
    816

Gene/Insert 2

  • Gene/Insert name
    tk
  • Insert Size (bp)
    1131
  • Promoter CAG

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    oct4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1317
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Gene/Insert 4

  • Gene/Insert name
    Klf4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    971
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 5

  • Gene/Insert name
    Sox2
  • Insert Size (bp)
    1610
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 6

  • Gene/Insert name
    myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter EF1a

Cloning Information for Gene/Insert 6

Gene/Insert 7

  • Gene/Insert name
    EBNA-1
  • Insert Size (bp)
    1923

Gene/Insert 8

  • Gene/Insert name
    Amp
  • Insert Size (bp)
    858

Gene/Insert 9

  • Gene/Insert name
    Lin28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2647
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Gene/Insert 10

  • Gene/Insert name
    Nanog
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    935
  • Entrez Gene
    NANOG
  • Promoter CAG

Cloning Information for Gene/Insert 10

Gene/Insert 11

  • Gene/Insert name
    IRES
  • Insert Size (bp)
    581

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMaster1 was a gift from Mario Capecchi (Addgene plasmid # 58525 ; http://n2t.net/addgene:58525 ; RRID:Addgene_58525)
  • For your References section:

    Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Wu S, Wu Y, Zhang X, Capecchi MR. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. 10.1073/pnas.1409933111 PubMed 25002522