pCAGGS-CD4-YEML-myc
(Plasmid #58538)

• Purpose: Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.
• Depositing Lab: Jacob Yount
• Publication: Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 10.1074/jbc.M114.557694. Epub 2014 Mar 13.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD4
• Species: H. sapiens (human)
• Mutation: Added C-terminal Tyrosine-Glutamate-Methionine-Leucine (YEML) motif followed by myc tag
• Entrez Gene: CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
• Tags / Fusion Proteins:
  • IFITM3 endocytosis Tyrosine-Glutamate-Methionine-Leucine (YEML) motif (C terminal on insert)
  • myc (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing has found an R318G amino acid residue substitution in CD4. The affect on protein function is not known.