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pFB-U6_CreOff-CMV-EGFP-shCtrl1
(Plasmid #58669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58669 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pFB-CMV-SV40
  • Total vector size (bp) 7512
  • Vector type
    Mammalian Expression, RNAi, Cre/Lox
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Control1 shRNA
  • gRNA/shRNA sequence
    GgaggatcaaattgatagtaaaccGTTTTGGCCACTGACTGACggtttactatcaatttgatcctcTTTTTG
  • Promoter U6
  • Tag / Fusion Protein
    • CMV-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLLU-5 cacagacttgtgggagaagc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene was unable to sequence verify the entire shRNA sequence due to the hairpin structure of the shRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-U6_CreOff-CMV-EGFP-shCtrl1 was a gift from Savio Chan (Addgene plasmid # 58669)
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