mouse ptc probe M2-3
(Plasmid #58701)

• Purpose: makes mouse ptc probe for in situ hybridization
• Depositing Lab: Matthew Scott
• Publication: Scott lab plasmids (unpublished)

Backbone

• Vector backbone: pBluescript II KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 3800
• Vector type: Bacterial Expression ; in situ probe

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ptc
• Species: M. musculus (mouse)
• Insert Size (bp): 841
• Mutation: only EcoRI fragment (5' end)
• Entrez Gene: Ptch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For sense: linearize with HindIII, transcribe with T7.
For anti-sense: linearize with BamHI, transcribe with T3.