mouse ptc probe M2-3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58701||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS+
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3800
Vector typeBacterial Expression ; in situ probe
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)841
Mutationonly EcoRI fragment (5' end)
Entrez GenePtch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
For sense: linearize with HindIII, transcribe with T7.
For anti-sense: linearize with BamHI, transcribe with T3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mouse ptc probe M2-3 was a gift from Matthew Scott (Addgene plasmid # 58701 ; http://n2t.net/addgene:58701 ; RRID:Addgene_58701)