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pET28a-ybbR-HIS-Xyl-DocI
(Plasmid #58711)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58711 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 6689
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Xyl-DocI
  • Alt name
    Xylanase T6
  • Alt name
    dockerin type I
  • Species
    Synthetic; Geobacillus stearothermophilus, Clostridium thermocellum
  • Insert Size (bp)
    1473
  • Mutation
    changed Threonin 120 to Cysteine in Xylanase Domain
  • Promoter T7
  • Tags / Fusion Proteins
    • ybbR (N terminal on insert)
    • HIS (N terminal on insert)
    • HRV 3C (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We thankfully received the xylanase and dockerin domain from Professor Edward Bayer (Department of Biological Chemistry, Weizmann Institute of Science, Israel) as recently described in this paper: Stahl, S. W., Nash, M. A., Fried, D. B., Slutzki, M., Barak, Y., Bayer, E. A., & Gaub, H. E. (2012). Single-molecule dissection of the high-affinity cohesin-dockerin complex. Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20431–6. doi:10.1073/pnas.1211929109
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-ybbR-HIS-Xyl-DocI was a gift from Hermann Gaub (Addgene plasmid # 58711 ; http://n2t.net/addgene:58711 ; RRID:Addgene_58711)
  • For your References section:

    From genes to protein mechanics on a chip. Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash MA, Gaub HE. Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099. 10.1038/nmeth.3099 PubMed 25194847