pUAST YFP Rab18 DN
(Plasmid
#58722)
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Purposeexpression of drosophila YFP Rab18 dominant negative mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUAST
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab18
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SpeciesD. melanogaster (fly)
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MutationS19N
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Entrez GeneRab18 (a.k.a. Dmel_CG3129, AAF46057, CG3129, DRABR4, DRabRP4, DmRab18, Dmel\CG3129, Rab-RP4, Rab-r4, Rab18/RP4, rab18)
- Promoter GAL4
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct contains a SNP - L8P.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST YFP Rab18 DN was a gift from Matthew Scott (Addgene plasmid # 58722 ; http://n2t.net/addgene:58722 ; RRID:Addgene_58722) -
For your References section:
Rab35 controls actin bundling by recruiting fascin as an effector protein. Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP. Science. 2009 Sep 4;325(5945):1250-4. doi: 10.1126/science.1174921. 10.1126/science.1174921 PubMed 19729655