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Purposeexpression of HA tagged rat Alpha2 delta-1 in mamalian cells
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Depositing Lab
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Depositing OrganizationUniversity College London
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58729-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58729-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha2 delta-1
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Alt nameCacna2d1
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Alt namecalcium channel, voltage-dependent, alpha2/delta subunit 1
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SpeciesR. norvegicus (rat)
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Entrez GeneCacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)
- Promoter SV40
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is an HA tag in middle of insert.
DNA (Catalog # 58729-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58729-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat Alpha2 delta-1 HA pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58729 ; http://n2t.net/addgene:58729 ; RRID:Addgene_58729) -
For your References section:
Calcium currents are enhanced by alpha2delta-1 lacking its membrane anchor. Kadurin I, Alvarez-Laviada A, Ng SF, Walker-Gray R, D'Arco M, Fadel MG, Pratt WS, Dolphin AC. J Biol Chem. 2012 Sep 28;287(40):33554-66. Epub 2012 Aug 6. 10.1074/jbc.M112.378554 PubMed 22869375
