Rat Alpha2 delta-1 mutMIDAS pMT2
(Plasmid
#58730)
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Purposeexpression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha2 delta-1
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Alt nameCacna2d1
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Alt namecalcium channel, voltage-dependent, alpha2/delta subunit 1
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SpeciesR. norvegicus (rat)
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MutationMIDAS site mutations (D259VSGS to AVAGA)
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Entrez GeneCacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)
- Promoter SV40
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat Alpha2 delta-1 mutMIDAS pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58730 ; http://n2t.net/addgene:58730 ; RRID:Addgene_58730) -
For your References section:
alpha2delta expression sets presynaptic calcium channel abundance and release probability. Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA. Nature. 2012 May 13;486(7401):122-5. doi: 10.1038/nature11033. 10.1038/nature11033 PubMed 22678293
