pAAV-Syn-CsChR-GFP
(Plasmid #58841)

• Purpose: AAV expression of CsChR-GFP under the Syn promoter
• Depositing Lab: Edward Boyden
• Publication: Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 4368
• Total vector size (bp): 6141
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C (use ampicillin if using DH5a)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CsChR-GFP
• Alt name: ChR87-GFP
• Alt name: Chloromonas subdivisa channelrhodopsin
• Species: Chloromonas subdivisa
• Insert Size (bp): 1773
• GenBank ID: KF992078
• Promoter: Syn
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CTGCGTATGAGTGCAAG
• 3′ sequencing primer: cagcgtatccacatagcg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is sequenced completely by the depositing lab except for part of the 5' ITR.