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(Plasmid #58893)


Item Catalog # Description Quantity Price (USD)
Plasmid 58893 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
  • GenBank ID
    NM_014786.3 NP_055601.2
  • Entrez Gene
    ARHGEF17 (a.k.a. P164RHOGEF, TEM4, p164-RhoGEF)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence identified a L722F mutation in TEM4. It is unknown whether this mutation affects protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP TEM4 FL was a gift from Channing Der (Addgene plasmid # 58893 ; ; RRID:Addgene_58893)
  • For your References section:

    Identification of a novel actin-binding domain within the Rho guanine nucleotide exchange factor TEM4. Mitin N, Rossman KL, Der CJ. PLoS One. 2012;7(7):e41876. doi: 10.1371/journal.pone.0041876. Epub 2012 Jul 24. 10.1371/journal.pone.0041876 PubMed 22911862