pCMV-Myc-XPLN
(Plasmid
#73365)
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PurposeExpresses myc tagged XPLN in mammalian cells
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Depositing Labs
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 73365-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 73365-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV-Myc
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Backbone manufacturerclontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namearhgef3
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Alt nameXPLN
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SpeciesH. sapiens (human)
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Entrez GeneARHGEF3 (a.k.a. GEF3, STA3, XPLN)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (QKLISEEDL) (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived as gift from Professor Krister Wennerberg (University of Helsinki, Helsinki).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original Publication: XPLN, a Guanine Nucleotide Exchange Factor for RhoA and RhoB, But Not RhoC
DNA (Catalog # 73365-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 73365-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Myc-XPLN was a gift from Jie Chen & Krister Wennerberg (Addgene plasmid # 73365 ; http://n2t.net/addgene:73365 ; RRID:Addgene_73365) -
For your References section:
XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC. Arthur WT, Ellerbroek SM, Der CJ, Burridge K, Wennerberg K. J Biol Chem. 2002 Nov 8;277(45):42964-72. Epub 2002 Sep 6. 10.1074/jbc.M207401200 PubMed 12221096