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pVk7-33G1-Flag
(Plasmid #58950)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58950 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size (bp) 5925
  • Modifications to backbone
    Kozak and leader of Vkappa 7-33, Gamma1 heavy constant region, C-terminal addition of monobiotinylation site, linker sequence and 6His
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG tag (DYKDDDDK) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVk7-33G1-Flag was a gift from Gavin Wright (Addgene plasmid # 58950)
  • For your References section:

    A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining. Staudt N, Muller-Sienerth N, Fane-Dremucheva A, Yusaf SP, Millrine D, Wright GJ. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. doi: 10.1016/j.bbrc.2014.11.123. Epub 2014 Dec 6. 10.1016/j.bbrc.2014.11.123 PubMed 25490391