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pGex-4T-1 Jip4 (1-272)
(Plasmid #59002)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGex-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5610
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mapk8ip4
  • Alt name
    Jip4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    710
  • Mutation
    Jip4 (aa1-272)
  • GenBank ID
    AY823270.1
  • Entrez Gene
    Spag9 (a.k.a. 3110018C07Rik, 4733401I23Rik, 4831406C20Rik, JIP-4, JLP, JSAP2, JSAP2a, Jip4, Mapk8ip4, syd1)
  • Tag / Fusion Protein
    • Gst (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer Gex 5'
  • 3′ sequencing primer Gex 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGex-4T-1 Jip4 (1-272) was a gift from Roger Davis (Addgene plasmid # 59002 ; http://n2t.net/addgene:59002 ; RRID:Addgene_59002)
  • For your References section:

    Role of the JIP4 scaffold protein in the regulation of mitogen-activated protein kinase signaling pathways. Kelkar N, Standen CL, Davis RJ. Mol Cell Biol. 2005 Apr;25(7):2733-43. 10.1128/MCB.25.7.2733-2743.2005 PubMed 15767678