P21-sp64 Poly A
(Plasmid #59433)

• Purpose: synthetic poly A transcripts of VZV
• Depositing Lab: Randall Cohrs
• Publication: Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Backbone

• Vector backbone: pSP64 poly(A)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3030
• Total vector size (bp): 6144
• Modifications to backbone: none
• Vector type: makes synthetic transcripts that contains poly A
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: none grows well, can extract sequencing grade plasmid from 1.5 ml of overnight culture.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VZV ORF 21
• Species: human herpesvirus type 3 (varicella zoster virus)
• Insert Size (bp): 3114
• Mutation: none
• GenBank ID: X04370.1
• Promoter: SP6
• Tag / Fusion Protein:
  • none (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HinD III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I cloned it (RJC)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry