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pCMV-OptoFGFR1
(Plasmid #59776)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59776 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCitrine-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 7550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OptoFGFR1
  • Alt name
    Lyn-cytoFGFR1-PHR-mCit
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    3617
  • GenBank ID
    NM_023110.2
  • Entrez Gene
    FGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCitrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-pAR
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-OptoFGFR1 was a gift from Won Do Heo (Addgene plasmid # 59776 ; http://n2t.net/addgene:59776 ; RRID:Addgene_59776)
  • For your References section:

    Spatiotemporal control of fibroblast growth factor receptor signals by blue light. Kim N, Kim JM, Lee M, Kim CY, Chang KY, Heo WD. Chem Biol. 2014 Jul 17;21(7):903-12. doi: 10.1016/j.chembiol.2014.05.013. Epub 2014 Jun 26. 10.1016/j.chembiol.2014.05.013 PubMed 24981772