Purpose(Empty Backbone) SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60503||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2021
Vector typeMammalian Expression ; Transposon
- Promoter TCE & RPBSA
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CCTGGAGCCAATTCCAACTCT
- 3′ sequencing primer CACTGCATTCTTGTTGTGGTT (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSBtet-RN was a gift from Eric Kowarz (Addgene plasmid # 60503 ; http://n2t.net/addgene:60503 ; RRID:Addgene_60503)
For your References section:Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Kowarz E, Loescher D, Marschalek R. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. 10.1002/biot.201400821 PubMed 25650551