DJ-1 TEV site pET15b
PurposeRecombinant protein expression of Park7
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60687||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5708
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt namePARK 7
SpeciesH. sapiens (human)
MutationTEV site was inserted (see comments below).
Entrez GenePARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
- Promoter T7
/ Fusion Protein
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This construct contains a Tobacco etch virus (TEV) protease recognition sequence (ENLYFQS) after the Thromin recognition site, and just before DJ-1. (Ex. LVPRGSHMENLYFQSASKRAL......)-TEV site italicized. Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DJ-1 TEV site pET15b was a gift from Mark Wilson (Addgene plasmid # 60687 ; http://n2t.net/addgene:60687 ; RRID:Addgene_60687)