|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60810||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Alt nameactivation-induced cytidine deaminase
SpeciesH. sapiens (human)
Mutationcodon optimized for yeast
Entrez GeneAICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
- Promoter GAL1
/ Fusion Protein
- Myc (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pESC-LEU-HsAIDSc was a gift from Thomas Kunkel & Youri Pavlov (Addgene plasmid # 60810 ; http://n2t.net/addgene:60810 ; RRID:Addgene_60810)
For your References section:Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes. Mayorov VI, Rogozin IB, Adkison LR, Frahm C, Kunkel TA, Pavlov YI. BMC Immunol. 2005 Jun 10;6:10. 10.1186/1471-2172-6-10 PubMed 15949042