pCRII HSPB7
(Plasmid
#61034)
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PurposeProbe for HSPB7 for in situ hybridization in zebrafish
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCRII
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHSPB7
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SpeciesD. rerio (zebrafish)
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GenBank IDBC083373
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Entrez Genehspb7 (a.k.a. zgc:103426)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
linearize with NotI, transcribe with SP6, probe length is 519.
Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII HSPB7 was a gift from Lara Hutson (Addgene plasmid # 61034 ; http://n2t.net/addgene:61034 ; RRID:Addgene_61034) -
For your References section:
Developmental expression patterns of the zebrafish small heat shock proteins. Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD. Dev Dyn. 2008 Feb;237(2):454-63. 10.1002/dvdy.21414 PubMed 18161059