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6his-WT gusA-pET28a+
(Plasmid #61156)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61156 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    INVaF'
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gusA
  • Alt name
    beta-glucuronidase
  • Species
    Escherichia coli
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Matsumura lab strain number 337

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6his-WT gusA-pET28a+ was a gift from Andrew Ellington & Ichiro Matsumura (Addgene plasmid # 61156 ; http://n2t.net/addgene:61156 ; RRID:Addgene_61156)
  • For your References section:

    Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase. Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD. Nat Biotechnol. 1999 Jul;17(7):696-701. 10.1038/10910 PubMed 10404164