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pmIL-6 mut C/EBP
(Plasmid #61291)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61291 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    Promega
  • Total vector size (bp) 6877
  • Modifications to backbone
    pGL2-Basic vector was digested with BglII and HindIII. The PCR product generated from primers mIL6BAM and mIL6HIND using CH12.LX genomic DNA as template was digested with BamHI and HindIII, and ligated into the digested pGL2-Basic vector which destroyed the 5' BglII/BamHI cloning site
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IL-6 promoter
  • Species
    M. musculus (mouse)
  • Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to ACAGATATCAATCT
  • Entrez Gene
    Il6 (a.k.a. Il-6)
  • Promoter IL-6 promoter with mutant C/EBP binding site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer EBV-rev
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains point mutations in C/EBP binding site. See Table 1 of associated publication for more details.

Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmIL-6 mut C/EBP was a gift from Gail Bishop (Addgene plasmid # 61291 ; http://n2t.net/addgene:61291 ; RRID:Addgene_61291)
  • For your References section:

    CD40-mediated transcriptional regulation of the IL-6 gene in B lymphocytes: involvement of NF-kappa B, AP-1, and C/EBP. Baccam M, Woo SY, Vinson C, Bishop GA. J Immunol. 2003 Mar 15;170(6):3099-108. PubMed 12626566