pmIL-6 mut NF-kB
Purposedrives luciferase from mouse IL-6 promoter with mutation in NF-kB site
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61293||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL2 basic
- Total vector size (bp) 6877
Modifications to backbonepGL2-Basic vector was digested with BglII and HindIII. The PCR product generated from primers mIL6BAM and mIL6HIND using CH12.LX genomic DNA as template was digested with BamHI and HindIII, and ligated into the digested pGL2-Basic vector which destroyed the 5' BglII/BamHI cloning site
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameIL-6 promoter
SpeciesM. musculus (mouse)
MutationMutated NF-kB binding site from GTGGGATTTTCCCA to GTGGGATTTTAGAC
Entrez GeneIl6 (a.k.a. Il-6)
- Promoter IL-6 promoter with mutant NF-kB binding site
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer EBV-rev
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Contains point mutations in NF-kB binding site. See Table 1 of associated publication for more details.
Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmIL-6 mut NF-kB was a gift from Gail Bishop (Addgene plasmid # 61293 ; http://n2t.net/addgene:61293 ; RRID:Addgene_61293)
For your References section:CD40-mediated transcriptional regulation of the IL-6 gene in B lymphocytes: involvement of NF-kappa B, AP-1, and C/EBP. Baccam M, Woo SY, Vinson C, Bishop GA. J Immunol. 2003 Mar 15;170(6):3099-108. PubMed 12626566