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pCWori cytochrome P450-BM3h 9D7
(Plasmid #61308)


Item Catalog # Description Quantity Price (USD)
Plasmid 61308 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6365
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Transform into BL21(DE3) for expression. Cultures can be grown at 37 degC until induction, after which the cultures should be incubated at 30 degC.
  • Copy number


  • Gene/Insert name
    Cytochrome P450-BM3 heme domain 9D7
  • Alt name
  • Alt name
  • Alt name
  • Species
    Bacillus megaterium
  • Insert Size (bp)
  • Mutation
    T268A, I263A, T438V, A328G
  • GenBank ID
    J04832.1 142797
  • Promoter Tac
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer LacI-R
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    We received this plasmid from Eric Brustad (first author of the article and the researcher who cloned the gene) as a result of the collaboration between our lab (Jasanoff Lab) and the Arnold Lab on the primary reference article.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWori cytochrome P450-BM3h 9D7 was a gift from Alan Jasanoff (Addgene plasmid # 61308 ; ; RRID:Addgene_61308)
  • For your References section:

    Structure-guided directed evolution of highly selective p450-based magnetic resonance imaging sensors for dopamine and serotonin. Brustad EM, Lelyveld VS, Snow CD, Crook N, Jung ST, Martinez FM, Scholl TJ, Jasanoff A, Arnold FH. J Mol Biol. 2012 Sep 14;422(2):245-62. doi: 10.1016/j.jmb.2012.05.029. Epub 2012 May 30. 10.1016/j.jmb.2012.05.029 PubMed 22659321