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pBW412hrpL-cIgfp
(Plasmid #61438)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61438 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSB4A3
  • Backbone manufacturer
    BioBrick registry
  • Backbone size w/o insert (bp) 3547
  • Total vector size (bp) 5429
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HrpL-rbs32-CI-LVA-t-plam-30gfp-t
  • Species
    Synthetic
  • Insert Size (bp)
    2090
  • Promoter HrpL
  • Tag / Fusion Protein
    • LVA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer TGCCACCTGACGTCTAAGAA
  • 3′ sequencing primer ATTACCGCCTTTGAGTGAGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBW412hrpL-cIgfp was a gift from Martin Buck & Baojun Wang (Addgene plasmid # 61438)
  • For your References section:

    Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Wang B, Kitney RI, Joly N, Buck M. Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516. 10.1038/ncomms1516 PubMed 22009040