PurposeHuman transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61547||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameTransferrin receptor
SpeciesH. sapiens (human)
Entrez GeneTFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
- Promoter CMV
/ Fusion Protein
- pHoran4 (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TfR-pHoran4 was a gift from David Perrais (Addgene plasmid # 61547 ; http://n2t.net/addgene:61547 ; RRID:Addgene_61547)
For your References section:pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. Shen Y, Rosendale M, Campbell RE, Perrais D. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. 10.1083/jcb.201404107 PubMed 25385186