PurposeHuman transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61547||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameTransferrin receptor
SpeciesH. sapiens (human)
Entrez GeneTFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
- Promoter CMV
/ Fusion Protein
- pHoran4 (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TfR-pHoran4 was a gift from David Perrais (Addgene plasmid # 61547)
For your References section:pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. Shen Y, Rosendale M, Campbell RE, Perrais D. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. 10.1083/jcb.201404107 PubMed 25385186
Generated by Addgene from full sequence supplied by depositor.