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1.3xWT HBV-Luciferase
(Plasmid #61549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61549 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 61549-D50 50 μg of DNA in Tris buffer $495
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DNA 61549-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGEM3Z
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1.3 times of the HBV genome with luciferase within the Core ORF
  • Insert Size (bp)
    3932
  • Mutation
    Substitution of HBV sequences with luciferase ORF. No vector mammalian promoters. HBV inherent enhancer and promoters

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing laboratory has indicated that this plasmid does not produce virus since it lacks the polymerase/RTase gene. It is recommend to consult with your institutions Biosafety Committee prior to use, as different institutions may have specific requirements for usage.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1.3xWT HBV-Luciferase was a gift from Yosef Shaul (Addgene plasmid # 61549 ; http://n2t.net/addgene:61549 ; RRID:Addgene_61549)

  • For your References section:

    Enhancer I predominance in hepatitis B virus gene expression. Doitsh G, Shaul Y. Mol Cell Biol. 2004 Feb;24(4):1799-808. PubMed 14749394
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