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pRSETb-pHlash
(Plasmid #61550)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61550 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSETb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 4580
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pHlash
  • Alt name
    Rluc8-cpVenus
  • Species
    Synthetic
  • Insert Size (bp)
    1680
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer AGGATCCAATGGCTTCCAAGGTGTACGA
  • 3′ sequencing primer CGCGAATTCTTACTCGATGTTGTGGCGGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fusion components were requested from collaborator sources. The complete vector was constructed by the submitting party.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETb-pHlash was a gift from Carl Johnson (Addgene plasmid # 61550 ; http://n2t.net/addgene:61550 ; RRID:Addgene_61550)
  • For your References section:

    pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH. Zhang Y, Xie Q, Robertson JB, Johnson CH. PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Epub 2012 Aug 14. 10.1371/journal.pone.0043072 PubMed 22905204