pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
(Plasmid #61629)

• Purpose: R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter
• Depositing Lab: Dolf Weijers
• Publication: Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2.

Backbone

• Vector backbone: pGreenII
• Backbone size w/o insert (bp): 2580
• Total vector size (bp): 12791
• Vector type: Plant Expression
• Selectable markers: Methothrexate

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mDII
• Alt name: Fragment of Arabidopsis IAA28, mutated to prevent auxin-dependent degradation
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 102
• Promoter: RPS5A

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13_forward20_primer

Gene/Insert 2

• Gene/Insert name: ntdTomato
• Species: Synthetic
• Insert Size (bp): 1518
• GenBank ID: KJ081243.1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: M13_forward20_primer

Gene/Insert 3

• Gene/Insert name: DII
• Alt name: Fragment of Arabidopsis IAA28
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 102
• Promoter: RPS5A

Cloning Information for Gene/Insert 3

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: dsRed1_C_primer

Gene/Insert 4

• Gene/Insert name: n3xVenus
• Alt name: nuclear 3xVenus
• Species: Synthetic
• Insert Size (bp): 2255

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: EGFP_N_primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry