PurposeBroad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62550||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
- Promoter trc
- Cloning method Unknown
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
See Nagant, et al. for an example of use of this plasmid (Microbiologyopen. 2013 Apr;2(2):318-25)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMF440 was a gift from Michael Franklin (Addgene plasmid # 62550 ; http://n2t.net/addgene:62550 ; RRID:Addgene_62550)